Histomorphological and Hormonal Changes in the Testes of Albino Rats Treated with Herbal Aphrodisiac Formular
Keywords:
Herbal Supplements, Histomorphological Changes, Hormonal Analysis, Testosterone Levels, Aphrodisiac FormulaAbstract
Herbal supplements, commonly referred to as botanicals, are plant-derived products utilized for their therapeutic properties. This study aims to examine the histomorphological and hormonal changes in the testes of albino rats treated with a herbal aphrodisiac formula. A total of eight male and two female rats were used in the study, with the males divided into control and test groups, while the females were used for mating with the test groups. Group A (Control) received only normal feed (growers’ mash) and distilled water daily for four weeks. Group B was administered 0.2 ml of Men’s Formula supplement and sacrificed on day 7, whereas Group C was administered 0.2 ml of Men’s Formula supplement and sacrificed on day 14. The body weights of the animals were measured before and after acclimatization, and similar weight assessments were performed at the end of each phase, with the average weights recorded accordingly. At the end of the procedure, the testes of each rat were excised under chloroform anesthesia and fixed in 10% formal saline for subsequent histological examination. Blood samples were collected via cardiac puncture into plain bottles, and sera were later separated for hormonal analysis. The results indicated that the herbal aphrodisiac formula enhanced testosterone levels and increased testis weight. However, no significant change (P > 0.05) was observed in body weight, and testicular histology remained unaffected. Further studies with prolonged usage of the herbal aphrodisiac formula are recommended to determine potential long-term effects
References
Bertolotto S, Heinrich M. Is the hype around the reproductive health claims of maca (Lepidium meyenii Walp.) justified? J Ethnopharmacol. 2020;211:126-70.
Boye JI, et al. Assessment of Polyscias fruticosa (L.) Harm (Araliaceae) leaf extract on male fertility in rats. J Ethnopharmacol 2018;215:164-171.
Buhner S. Natural Remedies for Low Testosterone: How to Enhance Male Sexual Health and Energy. 2nd ed. Boston: Healing Arts Press; 2016.
Chovanec M, Lauritsen J, Bandak M, Oing C, Kier GG, Kreiberg M, et al. Late adverse effects and quality of life in survivors of testicular germ cell tumour. Nat Rev Urol. 2021;18(4):227-45.
Chye N, et al. Traditional Asian folklore medicines in sexual health. J Tradit Chin Med 2006;26(4):266-273.
Clayton D, Gallagher PF, O’Mahony D. Explicit criteria as clinical tools to minimize inappropriate medication use and its consequences. Ther Adv Drug Saf. 2018;10:2042098619829431.
Defo A, et al. The methanolic extract of Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high-fat diet-induced obese sexually sluggish rats. J Ethnopharmacol 2017;209:258-265.
Defo PBD, Wankeu-Nya M, Ngadjui E, Fozin GRB, Kemka FX, Kamanyi A, et al. The methanolic extract of Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats. Asian Pac J Reprod. 2017;6(5):202-11.
Dutta S, Sengupta P. Medicinal herbs in the management of male infertility. Reprod Biol Endocrinol 2018;16(1):55.
El Keshky M, Rauf A, Walash M, et al. Male reproductive dysfunction: A global perspective. Reprod Health. 2020;17:48.
Fiorilli P, Zucchi A, Bellini P, et al. Psychosocial stress and male sexual health. J Psychosoc Res. 2019;12:25-30.
Herkommer P, Thibaut F, Bastos G, et al. Psychological and emotional effects of male sexual dysfunction. J Sex Med. 2024;21:257-67.
Jang Y, et al. The herbal formula KH-204 is protective against erectile dysfunction by minimizing oxidative stress and improving lipid profiles in a rat model of erectile dysfunction induced by hypercholesterolemia. Andrology 2017;5(4):745-754
Levin J, et al. Influence of testosterone on testicular function in male rats. Endocrinol 2016; 158(2):789-795.
Li L, Shi X, Shi Y, Wang Z. The signaling pathways involved in ovarian follicle development. Front Physiol. 2020;12:730196.
Mahajan G, et al. The antioxidant effects of herbal aphrodisiac formulas in male reproductive health. Phytother Res 2012;26(8):1231-1239.
Manikyam A. Social impacts of climate change mitigation policies and their implications for inequality. Clim Policy. 2024;19(7):827-44.
Mansour H, et al. Enhanced sperm parameters, serum hormonal levels, and testicular histology in Wistar rats after herbal supplementation. Reprod Biol Endocrinol 2022;20(1):25-32.
Michelini M, Olch A, Mihailidis D, et al. Tolerance limits and methodologies for IMRT measurement‐based verification QA: recommendations of AAPM Task Group No. 218. Med Phys. 2021;45(4):e53-e83.
Momen AL, Prieto N, de Alarcón R, et al. Management strategies for antidepressant-related sexual dysfunction: a clinical approach. J Clin Med. 2019;8(10):1640.
Msiska Z, et al. Herbal supplements and testosterone regulation: Effects on male sexual health and energy. Phytomedicine 2021;40:1-8.
Namoju S, et al. Phytoestrogens and phytosterols in the protection of testicular cells. J Herb Pharmacother 2021;21(2):94-105.
Pandit R, Albers G, Gilissen J, et al. Advance care planning in dementia: recommendations for healthcare professionals. BMC Palliat Care. 2019;17:1-17.
Prescott L, Logan E. The role of herbal medicine in male sexual health. Sex Med. 2019;7(4):144-58.
Rajendar B, Bharavi K, Rao GS, et al. Protective effect of an aphrodisiac herb Tribulus terrestris Linn on cadmium-induced testicular damage. Indian J Pharmacol. 2011;43(5):568-73.
Rajendar R, et al. Protective effect of Tribulus terrestris Linn on cadmium-induced testicular damage. Phytother Res 2011;25(7):1023-1031.
Raji Y, et al. Reproductive functions in male rats treated with methanolic extract of Alstonia boonei stem bark. Phytomedicine 2005;12(9):537-544.
Retzler A. Yohimbe: herbal remedy for erectile dysfunction. J Sex Med. 2019;16(2):85-95.
Teleanu P, Witt KL, Revanna P, et al. Next generation high throughput DNA damage detection platform for genotoxic compound screening. Sci Rep. 2022;8(1):1-20.
Wadden M, Nagpal TS, Barakat R. Impact of exercise during pregnancy on gestational weight gain and birth weight: an overview. Braz J Phys Ther. 2020;23(2):164-9.
Walsh G, Berman G. Metabolic actions of herbal supplements. J Nutr 2004;134(6):1314-1319.
Walsh KE, Berman JR. Sexual dysfunction in the older woman: an overview of the current understanding and management. Drugs Aging. 2004;21:655-75.
Welz L, Wu B, Ye J. Fatigue have impact on the sexual problems in Chinese females with systemic lupus erythematosus. BMC Women's Health. 2018;22(1):266.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Ajanwachukwu Ogbonna Wilson, Ohiwerei Wisdom Omogbai, Olugbenga Mary, Echekwube Marylyn E, Obeagu E I

This work is licensed under a Creative Commons Attribution 4.0 International License.